CD3 MOLCPX antibody

Catalog No. ABIN1176868

This Rat Monoclonal antibody specifically detects in FACS. It exhibits reactivity toward Mouse and has been mentioned in 2+ publications.

Quick Overview for CD3 MOLCPX antibody (ABIN1176868)

Target: CD3 MOLCPX

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Application: Flow Cytometry (FACS)

Clone: 17A2

Product Details

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: gammadelta TCR-positive T-T hybridoma D1

Isotype: IgG2b kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Mysliwietz, Thierfelder et al.: "Antilymphocytic antibodies and marrow transplantation. XII. Suppression of graft-versus-host disease by T-cell-modulating and depleting antimouse CD3 antibody is most effective when preinjected in ..." in: Blood, Vol. 80, Issue 10, pp. 2661-7, (1992) (PubMed).

Miescher, Schreyer, MacDonald: "Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex." in: Immunology letters, Vol. 23, Issue 2, pp. 113-8, (1990) (PubMed).

Target Details

Target: CD3 MOLCPX

Background: The 17A2 monoclonal antibody specifically binds to the T-cell receptor-associated CD3 complex that is expressed on many thymocytes and mature T lymphocytes. Plate-bound 17A2 antibody has been reported to induce IL-2 production by cultured T cells in the absence of accessory cells. The binding of the 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11. This suggests that the 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 antibody has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells.
Synonyms: CD3, CD3 epsilon, Cd3e, CD3epsilon, T3e